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KMID : 0613820200300100835
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Journal of Life Science
2020 Volume.30 No. 10 p.835 ~ p.843
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The Anti-obesity Effect of Aureobasidium pullulans SM-2001 Extract (Polycan®) on 3T3-L1 Preadipocytes and Adipocytes
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Kim Young-Suk
Lim Jong-Min
Ku Bon-Hwa
Moon Seung-Bae
Cho Hyung-Rae
Lee Seon-Min
Kwon Jung-Hee
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Abstract
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Obesity, the world’s leading metabolic disease, is a serious health problem in both industrialized and developing countries. Natural substances are of great interest in preventative medicine, especially in the field of metabolic syndromes?from insulin resistance to obesity and diabetes. In the present study, we investigated the effect of A. pullulans SM-2001 Extract (Polycan®) on the adipocyte differentiation of 3T3-L1 preadipocytes and the anti-obesity effect of 3T3-L1 adipocytes. Although β-glucan has been found to have health benefits in the regulation of the immune system and blood cholesterol levels, its role in obesity has not been fully investigated. Polycan® suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity without affecting cell viability in 3T3-L1 preadipocytes and adipocytes. Polycan® also inhibited cellular lipid accumulation through down-regulation of transcription factors, such as PPARγ and C/EBPα, and induced dose-dependent phosphorylation of AMP-activated protein kinase (AMPK)?a cellular energy sensor?while the total AMPK protein content remained unchanged. Taken together, this shows that the activation of AMPK by Polycan® in adipocytes plays a critical role in Polycan®-induced inhibition of adipocyte differentiation. Our results show that Polycan® has an anti-obesity action in vitro, suggesting a potential novel preventative agent for obesity and other metabolic diseases.
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KEYWORD
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Adipocyte differentiation, AMPK, beta-glucan, obesity, PPARγ
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